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| Registros recuperados : 2 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
18/03/2011 |
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Data da última atualização: |
15/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
NASCIMENTO, C. S. do; MACHADO, M. A.; GUIMARÃES, S. E. F.; GUIMARAES, M. F. M.; PEIXOTO, J. O.; FURLONG, J.; PRATA, M. C. de A.; VERNEQUE, R. da S.; TEODORO, R. L.; LOPES, P. S. |
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Afiliação: |
CARLOS SOUZA DO NASCIMENTO, UFV; MARCO ANTONIO MACHADO, CNPGL; SIMONE ELIZA FACIONI GUIMARÃES, UFV; MARTA FONSECA MARTINS GUIMARAES, CNPGL; J. OLIVEIRA PEIXOTO, UFV; JOHN FURLONG, CNPGL; MARCIA CRISTINA DE AZEVEDO PRATA, CNPGL; RUI DA SILVA VERNEQUE, CNPGL; ROBERTO LUIZ TEODORO, Pesquisador aposentado do CNPGL; PAULO SAVIO LOPES, UFV. |
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Título: |
Differential expression of genes in resistant versus susceptible Gyr x Holstein cattle challenged with the tick Rhipicephalus (Boophilus) microplus. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
Genetics and Molecular Research, v. 9, n. 4, p. 1974-1979, 2010. |
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DOI: |
https://doi.org/10.4238/vol9-4gmr905 |
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Idioma: |
Inglês |
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Conteúdo: |
The bovine tick Rhipicephalus (Boophilus) microplus causes major losses in cattle production systems in tropical regions. Bos indicus breeds are more resistant to ticks than B. taurus breeds. Resistance genes could be an alternative to control this parasite. We examined the pattern of gene expression of three calcium-binding-protein genes: translationally controlled tumor protein 1 (TPT1), allergen Bos d3 (S100A7), calcium channel protein transient receptor potential vanilloid 6 (TRPV6), and the cysteine proteinase inhibitor gene (CST6). These genes were selected from cDNA libraries prepared from skin biopsies taken from resistant and susceptible Gyr x Holstein F₂ animals. These biopsies were also used to study the expression level of these genes through real-time PCR analysis. The relative expression levels of the S100A7, TPT1, TRPV6, and CST6 genes were 2.01 ± 0.6, 1.32 ± 0.9, 1.53 ± 1.2, and 2.03 ± 0.7 times higher in the susceptible group, respectively. Skin lesion tissue from the susceptible animals showed significantly more mRNA transcripts of these genes in comparison with the resistant animals (P = 0.001). However, this hypersensitivity does not seem to protect the susceptible animals against tick infestation. |
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Palavras-Chave: |
CST6; QRT-PCR; S100A7; Tick resistance; TPT1; TRPV6. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/881545/1/Differential-expression-of-genes-in-resistant-versus-susceptible-Gyr-x-Holstein-cattle.pdf
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Marc: |
LEADER 02203naa a2200313 a 4500
001 1881545
005 2024-02-15
008 2010 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.4238/vol9-4gmr905$2DOI
100 1 $aNASCIMENTO, C. S. do
245 $aDifferential expression of genes in resistant versus susceptible Gyr x Holstein cattle challenged with the tick Rhipicephalus (Boophilus) microplus.$h[electronic resource]
260 $c2010
520 $aThe bovine tick Rhipicephalus (Boophilus) microplus causes major losses in cattle production systems in tropical regions. Bos indicus breeds are more resistant to ticks than B. taurus breeds. Resistance genes could be an alternative to control this parasite. We examined the pattern of gene expression of three calcium-binding-protein genes: translationally controlled tumor protein 1 (TPT1), allergen Bos d3 (S100A7), calcium channel protein transient receptor potential vanilloid 6 (TRPV6), and the cysteine proteinase inhibitor gene (CST6). These genes were selected from cDNA libraries prepared from skin biopsies taken from resistant and susceptible Gyr x Holstein F₂ animals. These biopsies were also used to study the expression level of these genes through real-time PCR analysis. The relative expression levels of the S100A7, TPT1, TRPV6, and CST6 genes were 2.01 ± 0.6, 1.32 ± 0.9, 1.53 ± 1.2, and 2.03 ± 0.7 times higher in the susceptible group, respectively. Skin lesion tissue from the susceptible animals showed significantly more mRNA transcripts of these genes in comparison with the resistant animals (P = 0.001). However, this hypersensitivity does not seem to protect the susceptible animals against tick infestation.
653 $aCST6
653 $aQRT-PCR
653 $aS100A7
653 $aTick resistance
653 $aTPT1
653 $aTRPV6
700 1 $aMACHADO, M. A.
700 1 $aGUIMARÃES, S. E. F.
700 1 $aGUIMARAES, M. F. M.
700 1 $aPEIXOTO, J. O.
700 1 $aFURLONG, J.
700 1 $aPRATA, M. C. de A.
700 1 $aVERNEQUE, R. da S.
700 1 $aTEODORO, R. L.
700 1 $aLOPES, P. S.
773 $tGenetics and Molecular Research$gv. 9, n. 4, p. 1974-1979, 2010.
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Embrapa Gado de Leite (CNPGL) |
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